Reagent Development Services: Proteins, Cytokines & Antibodies

PBL's reagent development services offer customized production of cytokines, interferons, antibodies, and other proteins.

 

Selected capabilities:

 

Protein Biophysical Characterization Service

 

With a wealth of knowledge on immunological interactions, we offer protein biophysical characterization services which can determine the kinetic characterization of protein-protein interactions. These services include determination of:

  • Affinity of protein-protein interactions
  • Association and dissociation rates of protein-protein interactions
  • Antibody-antigen specificity and affinity

PBL offers biophysical characterization of antibody affinity for a ligand or the measurement of antigen:antibody Kon/Koff rates. To accelerate assay development, we also determine which antibodies bind to antigens in a sandwich format. Binning— determination of antibodies which bind to non-overlapping epitopes—is performed using multiple hybridoma clones.

 

The graph below shows the association and dissociation of IFN to a surface-immobilized antibody as determined by real time Biolayer Interferometry (BLI) using varying concentrations of IFN. The raw data is shown in blue and the model response in red.

 

Figure 1. IFN Binding to and Dissociation from a Surface-Immobilized Monoclonal Antibody Evaluated by BLI

IFN Binding to and Dissociation from a Surface-Immobilized Monoclonal Antibody Evaluated by SPR

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